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factor κb ligand  (R&D Systems)


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    Structured Review

    R&D Systems factor κb ligand
    Factor κb Ligand, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 301 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ligand/Recombinant+Mouse+TRANCE%2FRANK+L%2FTNFSF11+(E%2E+coli-expressed)/pmc13085024-92-28-32
    Average 96 stars, based on 301 article reviews
    factor κb ligand - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: SPRY4-dependent ERK negative feedback demarcates functional adult stem cells in the male mouse germline†.
    Article Snippet: The differentiation medium (“cocktail”) included 1 μM RA with 25 ng/ml NRG1 (396-HB-050, R&D Systems) and 50 ng/ml Kit ligand (455-MC-010, R&D Systems), as indicated.

    Article Title: Single-cell morphodynamical trajectories enable prediction of gene expression accompanying cell state change
    Article Snippet: Seven different ligand conditions were tested at concentrations previously determined to elicit maximal cell responses (EGF 10 ng/ml (R&D Systems #236-EG), OSM 10 ng/ml (R&D Systems #8475-OM), TGFB 10 ng/ml (R&D Systems #240-B), EGF 10 ng/ml + OSM 10 ng/ml, TGFB 10 ng/ml + EGF 10 ng/ml, OSM 10 ng/ml + TGFB 10 ng/ml, TGFB 10 ng/ml + EGF 10 ng/ml + OSM 10 ng/ml).

    Article Title: SPRY4-dependent ERK negative feedback demarcates functional adult stem cells in the male mouse germline
    Article Snippet: The differentiation medium (“cocktail”) included 1 μM RA with 25 ng/ml NRG1 (396-HB-050, R&D Systems) and 50 ng/ml Kit ligand (455-MC-010, R&D Systems), as indicated.

    Microscopy:

    Article Title: Single-cell morphodynamical trajectories enable prediction of gene expression accompanying cell state change
    Article Snippet: .. Reporter cells were treated with ligand (EGF 10 ng/ml (R&D Systems #236-EG), OSM 10 ng/ml (R&D Systems #8475-OM), TGFB 10 ng/ml (R&D Systems #240-B), EGF 10 ng/ml + OSM 10 ng/ml, TGFB 10 ng/ml + EGF 10 ng/ml, OSM 10 ng/ml + TGFB 10 ng/ml, TGFB 10 ng/ml + EGF 10 ng/ml + OSM 10 ng/ml)and then imaged every 15 minutes for 48 hours with an Incucyte S3 microscope (1020×1280, 1.49 μm /pixel). ..

    Article Title: Single-cell morphodynamical trajectories enable prediction of gene expression accompanying cell state change.
    Article Snippet: .. Mills and from the same aliquot as the LINCS MCF10A dataset described above.65 The methodology used to generate the reporter cell line has been described previously.113 Reporter cells were treated with ligand (EGF 10 ng/ml (R&D Systems #236-EG), OSM 10 ng/ml (R&D Systems #8475-OM), TGFB 10 ng/ml (R&D Systems #240-B), EGF 10 ng/ml + OSM 10 ng/ml, TGFB 10 ng/ml + EGF 10 ng/ml, OSM 10 ng/ml + TGFB 10 ng/ml, TGFB 10 ng/ml + EGF 10 ng/ml + OSM 10 ng/ml)and then imaged every 15 minutes for 48 hours with an Incucyte S3 microscope (1020x1280, 1.49 μm/pixel). ..

    Binding Assay:

    Article Title: Human anti-IL-33 neutralizing monoclonal antibody
    Article Snippet: The Antibodies A and B bound to PEP12, the Antibodies C and D bound to PEP14, the Antibody E bound to both PEP16 and PEP17, the Antibody F bound to PEP24, and the Antibodies G and H bound to PEP26. .. No binding with the ligand was observed in the buffer alone or murine IgG (MAB002, available from R&D Systems, Inc.). ..

    Labeling:

    Article Title: A “Function-First” Approach to Identify Regulatory T cell–Targeting Antibodies for Immunotherapy
    Article Snippet: HEK293F cells were transiently transfected with WT mouse ICAM-1 (cDNA purchased from R&D Systems, cat. #RDC1449) for 24 hours prior to assay. .. Cells were treated with 10 μg/mL of unlabeled mAb or ligand (R&D Systems, cat:7987-AX-050) for 30 minutes at 4°C before the secondary fluorescently labeled detection agent (either PE-conjugated 12-D10 or anti-His to detect the ligand as indicated in ) was added for 30 minutes at 4°C. ..



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    Image Search Results


    Factors influencing the exhausted state of NK cells. The exhaustion of NK cells in the TME is regulated by multiple factors which results in NK cell function impairment due to: the downregulated expression of NK cell-activating receptors (e.g., NKG2D, DNAM-1, NKp30), the reduced secretion of effector cytokines (such as IFN-γ), and the upregulated expression of immunosuppressive molecules (e.g., PD-1, NKG2A, TIGIT, CD96, TIM-3, LAG-3). Additionally, transforming growth factor-β (TGF-β) secreted by tumor cells, cancer-associated fibroblasts (CAFs), and extracellular vesicles (EVs); hypoxic microenvironment; indoleamine 2,3-dioxygenase (IDO); prostaglandin E2 (PGE2); and immunosuppressive cells including macrophages, regulatory T cells (Treg), and myeloid-derived suppressor cells (MDSC) are involved in regulating NK cell exhaustion through multiple pathways.

    Journal: Pharmaceutical Science Advances

    Article Title: Mechanisms of tumor cell evasion from NK cell-mediated killing and advances in NK cell-based cancer immunotherapy

    doi: 10.1016/j.pscia.2026.100109

    Figure Lengend Snippet: Factors influencing the exhausted state of NK cells. The exhaustion of NK cells in the TME is regulated by multiple factors which results in NK cell function impairment due to: the downregulated expression of NK cell-activating receptors (e.g., NKG2D, DNAM-1, NKp30), the reduced secretion of effector cytokines (such as IFN-γ), and the upregulated expression of immunosuppressive molecules (e.g., PD-1, NKG2A, TIGIT, CD96, TIM-3, LAG-3). Additionally, transforming growth factor-β (TGF-β) secreted by tumor cells, cancer-associated fibroblasts (CAFs), and extracellular vesicles (EVs); hypoxic microenvironment; indoleamine 2,3-dioxygenase (IDO); prostaglandin E2 (PGE2); and immunosuppressive cells including macrophages, regulatory T cells (Treg), and myeloid-derived suppressor cells (MDSC) are involved in regulating NK cell exhaustion through multiple pathways.

    Article Snippet: TIM-3 ligands-including Galectin-9, PS, HMGB1, and CEACAM-1-play crucial roles in regulating NK activity.

    Techniques: Cell Function Assay, Expressing, Derivative Assay

    Strategies for enhancing NK cell anti-tumor activity. The anti-tumor efficacy of NK cells can be improved through multiple approaches: Adoptive NK cell infusions, including autologous NK cells (combined with HAIC), allogeneic NK cells (with high potential), and infusions with or without allogeneic transplantation; NK cell checkpoint inhibitors targeting molecules such as PD-1/PD-L1, KIRs (KIR2DL1/2/3/5), NKG2A/CD94, TIM-3, and LAG-3; Advanced applications of CAR-NK cells, including IL-15-secreting CAR-NK cell therapy targeting mesothelin, TIPE2 gene-knockout CAR-NK therapy, and application of modified CCCR-NK92; Administration of stimulatory cytokines like IL-2, IL-15, IL-18, and IL-21; Bi or trispecific killer engagers, including Bispecific antibody: LB1410 (anti-PD-1/TIM-3) and trispecific nanobody: PDL1/PD-1/NKG2A.

    Journal: Pharmaceutical Science Advances

    Article Title: Mechanisms of tumor cell evasion from NK cell-mediated killing and advances in NK cell-based cancer immunotherapy

    doi: 10.1016/j.pscia.2026.100109

    Figure Lengend Snippet: Strategies for enhancing NK cell anti-tumor activity. The anti-tumor efficacy of NK cells can be improved through multiple approaches: Adoptive NK cell infusions, including autologous NK cells (combined with HAIC), allogeneic NK cells (with high potential), and infusions with or without allogeneic transplantation; NK cell checkpoint inhibitors targeting molecules such as PD-1/PD-L1, KIRs (KIR2DL1/2/3/5), NKG2A/CD94, TIM-3, and LAG-3; Advanced applications of CAR-NK cells, including IL-15-secreting CAR-NK cell therapy targeting mesothelin, TIPE2 gene-knockout CAR-NK therapy, and application of modified CCCR-NK92; Administration of stimulatory cytokines like IL-2, IL-15, IL-18, and IL-21; Bi or trispecific killer engagers, including Bispecific antibody: LB1410 (anti-PD-1/TIM-3) and trispecific nanobody: PDL1/PD-1/NKG2A.

    Article Snippet: TIM-3 ligands-including Galectin-9, PS, HMGB1, and CEACAM-1-play crucial roles in regulating NK activity.

    Techniques: Activity Assay, Transplantation Assay, Gene Knockout, Modification

    Representative PD-L1 immunohistochemical images showing high expression (CPS=80). The brown DAB staining indicates PD-L1 expression on the cell membrane, while blue hematoxylin counterstain marks the nuclei (magnification, ×200; scale bar, 100 µm).

    Journal: Oncology Letters

    Article Title: Long survival of PD-L1-positive mediastinal sarcomatoid carcinoma after immunotherapy and anti-angiogenic target therapy: A case report

    doi: 10.3892/ol.2026.15690

    Figure Lengend Snippet: Representative PD-L1 immunohistochemical images showing high expression (CPS=80). The brown DAB staining indicates PD-L1 expression on the cell membrane, while blue hematoxylin counterstain marks the nuclei (magnification, ×200; scale bar, 100 µm).

    Article Snippet: The specimen obtained via bronchoscopy was sent to Amoy Diagnostics Co., Ltd. for genomic and programmed cell death ligand 1 (PD-L1) testing; no pathogenic gene mutations were detected.

    Techniques: Immunohistochemical staining, Expressing, Staining, Membrane